The Grass is Cleaner on the Other Side

From USDA:

Posted by Kelly Flynn, National Institute of Food and Agriculture, on December 9, 2014 at 2:00 PM

Research suggests that sorghum can be beneficial as both a fuel source and as a sinkhole for greenhouse gas. (iStock image)

This post is part of the Science Tuesday feature series on the USDA blog. Check back each week as we showcase stories and news from USDA’s rich science and research portfolio.

Liquid fuel, charcoal, and electric power are all possible byproducts of biomass feedstocks. But what if there was a feedstock that not only produced bioenergy, but acted as a greenhouse gas “sink” as well? According to Texas A&M’s AgriLife Research, there is: bioenergy sorghum.

Each region contains locally generated biomass feedstocks, ranging from grains to animal byproducts. Sorghum is a group of grasses with about 30 species, which can be used in a variety of bioenergy production processes, like starch-to-ethanol, sugar-to-ethanol, and plants-to-bioenergy.

Researchers from the university’s soil and crop sciences department made this discovery while measuring greenhouse gases from biofuel production lab experiments. The research helped quantify the carbon footprint of a bioenergy cropping system as part of the study, “Impacts of Biomass Sorghum Feedstock Production on Carbon Sequestration and Greenhouse Gas Emissions,” partially funded by the USDA’s National Institute of Food and Agriculture (NIFA).  The project received an Agriculture and Food Research Initiative grant in 2012.

According to the study, sorghum may play a significant role in future biofuel production as a high quality source. However, no studies have measured life-cycle greenhouse gases from bioenergy sorghum.

Analyzing the effects of crop rotation, nitrogen fertilization, and residue management were the main objectives. Researchers collected soil samples when the study began in 2008 and each spring to analyze the soil carbon storage and nutrient availability.

Annual accrual rates of soil organic carbon were much higher than expected. While promising, researchers said these rates may be due to the carbon-depleted soil where the experiments were conducted; severe drought conditions in 2010 and 2011 may have resulted in greater carbon production to aid roots searching for water.

“These results have significant implications for net greenhouse gas emissions, soil organic carbon sequestration and life-cycle analyses,” said Dr. Frank Hons, professor of soil science and AgriLife Research Faculty Fellow. “Few studies have quantified greenhouse gas emissions and below-ground carbon inputs from bioenergy sorghum, and further investigation is warranted.”

Through federal funding and leadership for research, education, and extension programs, NIFA focuses on investing in science and solving critical issues impacting people’s daily lives and the nation’s future. For more information, visit www.nifa.usda.gov.

USDA-Funded Researchers Map the Loblolly Pine Genome

USDA Blog Post:

Posted by Scott Elliott, National Institute of Food and Agriculture, on April 4, 2014 at 1:00 PM

During the month of April we will take a closer look at USDA’s Groundbreaking Research for a Revitalized Rural America, highlighting ways USDA researchers are improving the lives of Americans in ways you might never imagine, including research into trees that could fuel new energy solutions.

A team of researchers led by the University of California–Davis has mapped the complete genome of the loblolly pine. And if you don’t think that understanding the genetic makeup of loblolly pine is a big deal, perhaps you cannot see the forest for the trees.

Loblolly pine, the most commercially important tree in the United States, is the source of most paper products in this country and 58 percent of timber. On the surface, that might be reason enough for the USDA’s National Institute of Food and Agriculture (NIFA) to invest $14.6 million in 2011 toward science that could increase the productivity and health of American forests.

Loblolly pine also looms large on the horizon as a feedstock for the next generation of American biofuel.  President Obama’s goal of reducing the United States’ dependency on foreign oil by 30 percent by the year 2030 will be met, in large part, by producing home-grown biofuel. According to Genome Biology, approximately 75 percent of that biofuel will have to come from non-grain, non-food sources called lignocellulosic biomass – and loblolly pine could be a major contributor to filling that need.

Mapping the loblolly genome, then, became an important part of the plan in terms of improving the health and sustainability of this important plant. But, mapping a genome is no easy task, and the loblolly pine proved to be the greatest challenge to date for this type of research. The loblolly genome is the largest ever sequenced and is about seven times larger than the human genome.

To sequence a genome, scientists must first examine the DNA of their subject and then “map” the location of each nucleotide (the “A, C, T, and G” bases) of the entire DNA chain. Scientists use this information to find the best traits, such as disease resistance, and develop better future generations.

The challenge of overcoming the sheer volume of loblolly data is a triumph of its own for the research team.  According to David Neal, team leader and professor of plant sciences at UC–Davis, the team could “read” the nucleotide letters, but only in short batches. The problem was putting together the 16 billion fragments in a way that would allow them to read the complete story of the loblolly pine.

Researchers met this task by employing a new technique developed at the University of Maryland.  Team members overlapped smaller sections of data to form larger chunks and then threw away the redundant information. The process eventually meant the computer had 100 times less sequence data to deal with. The success of this process may help speed up future genome-mapping projects.

The loblolly project team consisted of UC–Davis, Johns Hopkins University, the University of Maryland, Indiana University–Bloomington, Texas A&M University, Children’s Hospital Oakland Research Institute, and Washington State University.

Their complete articles of this research were published in the March 2014 issues of GENETICS and Genome Biology.